Establishment of a T(m)-shift Method for Detection of Cat-Derived Hookworms
- Author:
Yeqi FU
1
;
Yunqiu LIU
;
Asmaa M I ABUZEID
;
Yue HUANG
;
Xue ZHOU
;
Long HE
;
Qi ZHAO
;
Xiu LI
;
Jumei LIU
;
Rongkun RAN
;
Guoqing LI
Author Information
- Publication Type:Original Article
- Keywords: Anoylostoma ceylanicum; Anoylostoma tubaeforme; cat; T(m)-shift; SNP; ITS1
- MeSH: Ancylostoma; Ancylostomatoidea; Animals; Cats; DNA; Freezing; Methods; Plasmids; Polymorphism, Single Nucleotide; Real-Time Polymerase Chain Reaction; Tail
- From:The Korean Journal of Parasitology 2019;57(1):9-15
- CountryRepublic of Korea
- Language:English
- Abstract: Melting temperature shift (T(m)-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a T(m)-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5′ end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of T(m)-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The T(m)-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of T(m)-values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10⁻⁶ and 5.28×10⁻⁶ ng/μl samples of AceP and AtuP, respectively. The accuracy of T(m)-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the T(m)-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.
