Comparison of Two PCR Assays for Trichomonas vaginalis
- Author:
Chang Suk NOH
1
;
Sang Su KIM
;
Sung Yul PARK
;
Hong Sang MOON
;
Yeonchul HONG
;
Jae Sook RYU
Author Information
- Publication Type:Brief Communication
- Keywords: Trichomonas vaginalis; PCR; DNA isolation
- MeSH: DNA; Humans; Male; Methods; Polymerase Chain Reaction; Prostatic Diseases; Trichomonas vaginalis; Trichomonas
- From:The Korean Journal of Parasitology 2019;57(1):27-31
- CountryRepublic of Korea
- Language:English
- Abstract: PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
