Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis
- Author:
Tahali MENDOZA-OLIVEROS
1
;
Victor ARANA-ARGÁEZ
;
Leidi C ALVARÉZ-SÁNCHEZ
;
Julio LARA-RIEGOS
;
María Elizbeth ALVARÉZ-SÁNCHEZ
;
Julio C TORRES-ROMERO
Author Information
- Publication Type:Brief Communication
- Keywords: Trichomonas vaginalis; TRPV channel; recombinant protein; immune response; BALB/c mouse
- MeSH: Animals; Calcium; Clone Cells; Coculture Techniques; Cytokines; Escherichia coli; Immunoglobulin G; In Vitro Techniques; Infection Control; Interleukin-10; Interleukin-6; Macrophages; Mice; Nickel; Parasites; Sexually Transmitted Diseases; T-Lymphocytes; Trichomonas vaginalis; Trichomonas
- From:The Korean Journal of Parasitology 2019;57(1):33-38
- CountryRepublic of Korea
- Language:English
- Abstract: Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4⁺ T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.
