Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
- Author:
Liu TIANLI
1
;
Wang XIFENG
;
Tian ZHENZHONG
;
Wang LIXIA
;
Zhang XINGXING
;
Qiao JUN
;
Meng QINGLING
;
Gong SHASHA
;
Chen YING
;
Cai XUEPENG
Author Information
- Publication Type:Brief Communication
- Keywords: multi-epitope; fusion protein; serodiagnostic; antigen; cystic echinococcosis; indirect ELISA; sheep
- MeSH: Animals, Domestic; Echinococcosis; Echinococcus granulosus; Enzyme-Linked Immunosorbent Assay; Epitopes, B-Lymphocyte; Methods; Parasitic Diseases; Sensitivity and Specificity; Serologic Tests; Sheep
- From:The Korean Journal of Parasitology 2019;57(1):61-67
- CountryRepublic of Korea
- Language:English
- Abstract: Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
