Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii
10.3347/kjp.2018.56.6.553
- Author:
Eun Kyung MOON
1
;
Hyun Seo CHOI
;
So Min PARK
;
Hyun Hee KONG
;
Fu Shi QUAN
Author Information
1. Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea. fquan01@gmail.com
- Publication Type:Original Article
- Keywords:
Acanthamoeba castellanii;
secretory product
- MeSH:
Acanthamoeba castellanii;
Acanthamoeba Keratitis;
Acanthamoeba;
Blindness;
Carboxylesterase;
Choline Dehydrogenase;
Chromatography, Liquid;
Electrophoresis, Polyacrylamide Gel;
Encephalitis;
Extracellular Space;
Eye Infections;
Keratitis;
Mass Spectrometry;
Peptide Hydrolases;
Virulence;
Vision Disorders
- From:The Korean Journal of Parasitology
2018;56(6):553-558
- CountryRepublic of Korea
- Language:English
-
Abstract:
Pathogenic Acanthamoeba spp. cause granulomatous amoebic encephalitis and keratitis. Acanthamoeba keratitis (AK) is a rare but serious ocular infection that can result in permanent visual impairment or blindness. However, pathogenic factors of AK remain unclear and treatment for AK is arduous. Expression levels of proteins secreted into extracellular space were compared between A. castellanii pathogenic (ACP) and non-pathogenic strains. Two-dimensional polyacrylamide gel electrophoresis revealed 123 differentially expressed proteins, including 34 increased proteins, 7 qualitative increased proteins, 65 decreased proteins, and 17 qualitative decreased proteins in ACP strain. Twenty protein spots with greater than 5-fold increase in ACP strain were analyzed by liquid chromatography triple quadrupole mass spectrometry. These proteins showed similarity each to inosine-uridine preferring nucleoside hydrolase, carboxylesterase, oxygen-dependent choline dehydrogenase, periplasmic-binding protein proteinases and hypothetical proteins. These proteins expressed higher in ACP may provide some information to understand pathogenicity of Acanthamoeba.
