Morphological and Molecular Characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis
10.3347/kjp.2018.56.6.567
- Author:
Umanets ALEXANDER
1
;
Chae Wong LIM
;
Bumseok KIM
;
Eui Ju HONG
;
Hyeon Cheol KIM
;
Bae Keun PARK
Author Information
1. College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea.
- Publication Type:Original Article
- Keywords:
Nyctereutes procyonoides koreensis;
Toxocara tanuki;
morphological and molecular characterization;
larva migrans;
Korean raccoon dog;
Korea
- MeSH:
Adult;
Base Sequence;
Female;
Humans;
Korea;
Larva;
Larva Migrans;
Male;
Raccoon Dogs;
Raccoons;
Republic of Korea;
Toxocara
- From:The Korean Journal of Parasitology
2018;56(6):567-575
- CountryRepublic of Korea
- Language:English
-
Abstract:
Present study was performed to describe the morphological and molecular characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean raccoon dog, Nyctereutes procyonoides koreensis, naturally infected in the Republic of Korea (Korea). Juvenile and adult worms of T. tanuki were recovered in 5 out of 10 raccoon dogs examined and the larval worms were detected in 15 out of 20 muscle samples (75%). Small lateral alae were observed on the cranial end of the body in male and female adults and 2 long spicules (3.0–3.5 mm) were characteristically observed in the posterior end of males. In SEM observation, 18 pairs of proximal precloacal, a precloacal median, a postcloacal median and 5 pairs of postcloacal papillae were uniquely revealed in the posterior portion of males, but the proximal papillae were not shown in the lateral ends of females. Molecular analysis on the 18S rRNA partial DNA sequences was revealed the same finding in both samples, adult worms and muscle larvae, which are closely related to T. tanuki. In conclusion, it was confirmed for the first time that T. tanuki is indigenously distributed, the Korean raccoon dog is acted as the natural definitive host of this nematode in Korea and the morphological characteristics of T. tanuki were shown in specific structure for single postcloacal median papilla in male.
