Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
10.3347/kjp.2018.56.5.437
- Author:
Won Gi YOO
1
;
Sun Min KIM
;
Eun Jeong WON
;
Ji Yun LEE
;
Fuhong DAI
;
Ho Choon WOO
;
Ho Woo NAM
;
Tae Im KIM
;
Jeong Hee HAN
;
Dongmi KWAK
;
Yun Sang CHO
;
Seung Won KANG
;
Tong Soo KIM
;
Xing Quan ZHU
;
Chunren WANG
;
Heejeong YOUN
;
Sung Jong HONG
Author Information
1. Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea. hongsj@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Toxoplasma gondii;
ELISA;
pork;
tissue fluid;
Korean;
imported
- MeSH:
Agriculture;
Antibodies;
Enzyme-Linked Immunosorbent Assay;
Korea;
Meat;
Prevalence;
Red Meat;
Sensitivity and Specificity;
Shoulder;
Social Control, Formal;
Toxoplasma
- From:The Korean Journal of Parasitology
2018;56(5):437-446
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066–0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
