Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China
10.3347/kjp.2018.56.4.391
- Author:
Bin YAN
1
;
Xiafei LIU
;
Junyuan WU
;
Shanshan ZHAO
;
Wumei YUAN
;
Baoju WANG
;
Hazi WURELI
;
Changchun TU
;
Chuangfu CHEN
;
Yuanzhi WANG
Author Information
1. School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832002, People’s Republic of China. wangyuanzhi621@126.com
- Publication Type:Brief Communication
- Keywords:
Echinococcus granulosus;
CO1 gene;
cystic echinococcosis;
northwest China
- MeSH:
Abattoirs;
Animals;
Argentina;
Australia;
Cattle;
China;
Clone Cells;
Cloning, Organism;
Echinococcosis;
Echinococcus granulosus;
Echinococcus;
France;
Genetic Variation;
Genotype;
Haploidy;
Haplotypes;
Helminths;
Humans;
Liver;
Livestock;
Middle East;
Polymerase Chain Reaction;
Sheep
- From:The Korean Journal of Parasitology
2018;56(4):391-396
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.
