Immune Responses Induced by HSP60 DNA Vaccine against Toxoplasma gondii Infection in Kunming Mice
10.3347/kjp.2018.56.3.237
- Author:
Zhong Yuan LI
1
;
Jing LU
;
Nian Zhang ZHANG
;
Jia CHEN
;
Xing Quan ZHU
Author Information
1. College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province 230036, P. R. China.
- Publication Type:Original Article
- Keywords:
Toxoplasma gondii;
HSP60 DNA;
Kunming mouse;
immune response
- MeSH:
Animals;
Antibodies;
Antigen-Presenting Cells;
Brain;
Chaperonin 60;
Cytokines;
DNA;
Humans;
Immunization;
Mice;
Parasites;
Spleen;
T-Lymphocytes;
Toxoplasma;
Toxoplasmosis;
Vertebrates
- From:The Korean Journal of Parasitology
2018;56(3):237-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii can infect all the vertebrates including human, and leads to serious toxoplasmosis and considerable veterinary problems. T. gondii heat shock protein 60 (HSP60) is associated with the activation of antigen presenting cells by inducing initial immune responses and releasing inflammatory cytokines. It might be a potential DNA vaccine candidate for this parasite. A pVAX-HSP60 DNA vaccine was constructed and immune responses was evaluated in Kunming mice in this study. Our data indicated that the innate and adaptive immune responses was elicited by successive immunizations with pVAX-HSP60 DNA, showing apparent increases of CD3e+CD4+ and CD3e+CD8a+ T cells in spleen tissues of the HSP60 DNA-immunized mice (24.70±1.23% and 10.90±0.89%, P < 0.05) and higher levels of specific antibodies in sera. Furthermore, the survival period of the immunized mice (10.53±4.78 day) were significantly prolonged during the acute T. gondii infection. Decrease of brain cysts was significant in the experimental group during the chronic infection (P < 0.01). Taken together, TgHSP60 DNA can be as a vaccine candidate to prevent the acute and chronic T. gondii infections.
