Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis
10.3347/kjp.2018.56.2.113
- Author:
Samah S ABDEL GAWAD
1
;
Mousa A M ISMAIL
;
Naglaa F A IMAM
;
Ahmed H A EASSA
;
Enas Yahia ABU-SAREA
Author Information
1. Parasitology Department, Faculty of Medicine, Beni-Suef University, Egypt.
- Publication Type:Original Article
- Keywords:
Cryptosporidium;
Beni-Suef;
PCR;
ELISA;
modified Ziehl-Neelsen stain
- MeSH:
Animals;
Cryptosporidiosis;
Cryptosporidium;
Diagnosis;
DNA;
Egypt;
Enzyme-Linked Immunosorbent Assay;
Epidemiologic Factors;
Epidemiology;
Humans;
Methods;
Microscopy;
Oocysts;
Polymerase Chain Reaction;
Water
- From:The Korean Journal of Parasitology
2018;56(2):113-119
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20–40 year-old group, followed by 40–60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.
