Serodiagnosis of Extraintestinal Amebiasis: Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit
- Author:
Nicolas BEYLS
1
;
Odile COGNET
;
Jean Paul STAHL
;
Olivier ROGEAUX
;
Herve PELLOUX
Author Information
- Publication Type:Brief Communication
- Keywords: Entamoeba histolytica; amebiasis; diagnosis; ELISA
- MeSH: Amebiasis; Antibodies; Axenic Culture; Diagnosis; Entamoeba histolytica; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Humans; Mass Screening; Methods; Retrospective Studies; Sensitivity and Specificity; Serologic Tests
- From:The Korean Journal of Parasitology 2018;56(1):71-74
- CountryRepublic of Korea
- Language:English
- Abstract: Soluble antigens from an axenic culture of Entamoeba histolytica were used to develop a commercial ELISA kit to quantify anti-E. histolytica antibodies in sera of patients with extraintestinal amebiasis in non-endemic settings. The diagnostic specificity and sensitivity of the test were assessed retrospectively using 131 human serum samples with amoebic serologic status available. They were selected according to their results in immunofluorescence (IFAT) and were separated in 2 sample categories: 64 sera with positive results by IFAT and 67 with negative results by IFAT. The sensitivity and specificity of the ELISA kit were assessed at 95.0% and 94.0% compared to the IFAT. The test can be useful to exclude a potential diagnosis of amebiasis and could be used as a screening method since ELISA is an automated technique.
