Analysis of osteogenic potential on 3mol% yttria-stabilized tetragonal zirconia polycrystals and two different niobium oxide containing zirconia ceramics
10.4047/jap.2018.10.2.147
- Author:
Aung Thu HEIN
1
;
Young Dan CHO
;
Ye Hyeon JO
;
Dae Joon KIM
;
Jung Suk HAN
Author Information
1. Department of Prosthodontics, School of Dentistry, Seoul National University, Seoul, Republic of Korea. proshan@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Dental implant;
Niobium;
Zirconia;
Low temperature degradation (LTD);
Osteogenic potential
- MeSH:
Alkaline Phosphatase;
Animals;
Cell Differentiation;
Cell Proliferation;
Ceramics;
Dental Implants;
Gene Expression;
Mice;
Niobium;
Osteoblasts;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Titanium
- From:The Journal of Advanced Prosthodontics
2018;10(2):147-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was performed to evaluate the osteogenic potential of 3mol% yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP) and niobium oxide containing Y-TZPs with specific ratios, new (Y,Nb)-TZPs, namely YN4533 and YN4533/Al20 discs. MATERIALS AND METHODS: 3Y-TZP, YN4533 and YN4533/Al20 discs (15 mm diameter and 1 mm thickness) were prepared and their average surface roughness (Ra) and surface topography were analyzed using 3-D confocal laser microscope (CLSM) and scanning electron microscope (SEM). Mouse pre-osteoblast MC3T3-E1 cells were seeded onto all zirconia discs and evaluated with regard to cell attachment and morphology by (CLSM), cell proliferation by PicoGreen assay, and cell differentiation by Reverse-Transcription PCR and Quantitative Real-Time PCR, and alkaline phosphatase (Alp) staining. RESULTS: The cellular morphology of MC3T3-E1 pre-osteoblasts was more stretched on a smooth surface than on a rough surface, regardless of the material. Cellular proliferation was higher on smooth surfaces, but there were no significant differences between 3Y-TZP, YN4533, and YN4533/Al20. Osteoblast differentiation patterns on YN4533 and YN4533/Al20 were similar to or slightly higher than seen in 3Y-TZP. Although there were no significant differences in bone marker gene expression (alkaline phosphatase and osteocalcin), Alp staining indicated better osteoblast differentiation on YN4533 and YN4533/Al20 compared to 3Y-TZP. CONCLUSION: Based on these results, niobium oxide containing Y-TZPs have comparable osteogenic potential to 3Y-TZP and are expected to be suitable alternative ceramics dental implant materials to titanium for aesthetically important areas.
