Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
10.24171/j.phrp.2019.10.1.06
- Author:
Mahmoud KHODABANDEH
1
;
Mohsen MOHAMMADI
;
Mohammad Reza ABDOLSALEHI
;
Azadeh ALVANDIMANESH
;
Mehrdad GHOLAMI
;
Meysam Hasannejad BIBALAN
;
Abazar POURNAJAF
;
Ramin KAFSHGARI
;
Ramazan RAJABNIA
Author Information
1. Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
- Publication Type:Original Article
- Keywords:
drug resistance;
methicillin-resistant S. aureus;
inducible clindamycin resistance
- MeSH:
Bacteria;
Clindamycin;
Diffusion;
Drug Resistance;
Genotype;
Lincosamides;
Macrolides;
Methicillin-Resistant Staphylococcus aureus;
Methods;
Phenotype;
Prevalence;
Staphylococcus aureus;
Staphylococcus;
Streptogramin B;
Streptogramins
- From:
Osong Public Health and Research Perspectives
2019;10(1):25-31
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples. METHODS: A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates. RESULTS: The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively. CONCLUSION: Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.
