Anti-inflammatory Activity of Standardized Fraction from Inula helenium L. via Suppression of NF-κB Pathway in RAW 264.7 Cells
10.20307/nps.2019.25.1.16
- Author:
Jaemoo CHUN
1
;
Kwangho SONG
;
Yeong Shik KIM
Author Information
1. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea. kims@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Inula helenium L.;
Sesquiterpene lactones;
Anti-inflammation;
NF-κB;
RAW 264.7 cells
- MeSH:
Carrageenan;
Cyclooxygenase 2;
Edema;
Inflammation;
Inula;
Lactones;
Methylene Chloride;
Nitric Oxide;
Nitric Oxide Synthase;
RAW 264.7 Cells;
Staphylococcal Protein A;
Water
- From:Natural Product Sciences
2019;25(1):16-22
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inula helenium L. is rich source of eudesmane-type sesquiterpene lactones, mainly alantolactone and isoalantolactone, which have the various pharmacological functions. In this study, we examined the inhibitory effects of nitric oxide (NO) production of hexane, methylene chloride, ethyl acetate, butanol, and water fractions from I. helenium and investigated the anti-inflammatory effect of hexane fraction of I. helenium (HFIH) in LPS-induced RAW 264.7 cells. Quantification of alantolactone and isoalantolactone from HFIH was carried out for the standardization by multiple reaction monitoring using triple quadrupole mass spectrometer. HFIH significantly inhibited inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) protein as well as their downstream products NO and prostaglandin E₂ (PGE₂) in LPS-stimulated RAW 264.7 cells. Moreover, HFIH suppressed NF-κB transcriptional activity by decreasing the translocation of p65 to the nucleus. The in vivo study further confirmed that HFIH attenuated the paw edema induced by carrageenan in an acute inflammation model. These findings suggest that HFIH may be useful as a promising phytomedicine for inflammatory-associated diseases.
