Chemical Composition and Antimicrobial Efficiency of Swietenia macrophylla Seed Extract on Clinical Wound Pathogens
10.20307/nps.2019.25.1.38
- Author:
Hanan Kumar GOPALAN
1
;
Nor Faizzah MD HANAFIAH
;
Leong CHEAN RING
;
Wen Nee TAN
;
Suzana WAHIDIN
;
Teo Siew HWAY
;
Tong Woei YENN
Author Information
1. Universiti Kuala Lumpur, Institute of Medical Science Technology, A1-1, Jalan TKS 1, Taman Kajang Sentral, Selangor, 43000 Kajang, Selangor, Malaysia.
- Publication Type:Original Article
- Keywords:
Swietenia macrophylla;
seed extract;
chemical composition;
antimicrobial activity
- MeSH:
Bacillus subtilis;
Bacteria;
Bandages;
Diffusion;
Gas Chromatography-Mass Spectrometry;
Gram-Negative Bacteria;
Hospitalization;
Linoleic Acid;
Meliaceae;
Methanol;
Methods;
Oleic Acid;
Silver;
Wound Infection;
Wounds and Injuries;
Yeasts
- From:Natural Product Sciences
2019;25(1):38-43
- CountryRepublic of Korea
- Language:English
-
Abstract:
Microbial wound infection prolonged the hospitalization and increase the cost for wound management. Silver is commonly used as antimicrobial wound dressing. However, it causes several adverse side effects. Hence, this study was aimed to evaluate the antimicrobial efficiency of Swietenia macrophylla seed extract on clinical wound pathogens. Besides, the bioactive constituents of the seed extract were also determined. S. macrophylla seeds were extracted with methanol by maceration method. The seed extract inhibited 5 test bacteria and 1 yeast on disc diffusion assay. The antibacterial activity was broad spectrum, as the extract inhibited both Gram positive and Gram negative bacteria. On kill curve analysis, the antibacterial activity of the seed extract was concentration-dependent, the increase of extract concentration resulted in more reduction of bacterial growth. The extract also caused 99.9% growth reduction of Bacillus subtilis relative to control. A total of 21 compounds were detected in gas chromatography-mass spectrometry analysis. The predominant compounds present in the extract were oleic acid (18.56%) and linoleic acid (17.72%). In conclusion, the methanolic extract of S. macrophylla seeds exhibited significant antimicrobial activity on clinical wound pathogens. Further investigations should be conducted to purify other bioactive compounds from the seeds of S. macrophylla.
