Study on the Marker Steroids of New Zealand Deer (Cervus elaphus var. scoticus) Velvet Antler by UPLC-MS/MS and HPLC-PDA Methods
10.20307/nps.2019.25.1.49
- Author:
Nam Kyung LEE
1
;
Kyoung Hwa JANG
;
Jong Tae LEE
;
Hee Won PARK
;
Sung Tai HAN
;
Gyo IN
Author Information
1. Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp., 30, Gajeong-ro, Yuseong-gu, Daejeon 34128, Republic of Korea. leenamkyung@kgc.co.kr
- Publication Type:Original Article
- Keywords:
New Zealand deer antler;
marker steroid;
UPLC-MS/MS;
HPLC-PDA;
steroid hormone;
7-oxycholesterol
- MeSH:
Androsterone;
Animals;
Antlers;
Chromatography, Liquid;
Deer;
Dehydroepiandrosterone;
Estrone;
Medroxyprogesterone;
Megestrol Acetate;
Methods;
New Zealand;
Progesterone;
Steroids;
Testosterone
- From:Natural Product Sciences
2019;25(1):49-58
- CountryRepublic of Korea
- Language:English
-
Abstract:
Eleven steroid hormones (SHs: androstene-3,17-dione, estrone, β-estradiol, α-estradiol, testosterone, dehydroepiandrosterone, 17á-hydroxyprogesterone, medroxyprogesterone, megestrol acetate, progesterone, and androsterone) were detected from New Zealand deer (Cervus elaphus var. scoticus) velvet antler (NZA, 鹿茸). A method for the quantification of eleven SHs was established by using ultraperformance liquid chromatography (UPLC)-MS/MS. The linearities (R² > 0.991), limits of quantification (LOQ values, 0.3 ng/mL to 23.1 ng/mL), intraday and interday precisions (relative standard deviation: RSD < 2.43%), and recovery rates (97.3% to 104.6%) for all eleven SHs were determined. In addition, a method for the quantification of three 7-oxycholesterols (7-O-CSs: 7-ketocholesterol, 7α-hydroxycholesterol, and 7β-hydroxycholesterol) in the NZA was established by using an HPLC-photodiode array (PDA) method. The linearities (R² > 0.999), LOQ values (30 ng/mL to 350 ng/mL), intraday and interday precisions (RSD < 1.93%), and recovery rates (97.2% to 103.5%) for the three 7-O-CSs were determined. These quantitative methods are accurate, precise, and reproducible. As a result, it is suggested that the five steroid compounds of androstene-3,17-dione, androsterone, 7-ketocholesterol, 7α-hydroxycholesterol, and 7β-hydroxycholesterol could be marker steroids of NZA. These methods can be applied to quantify or standardize the marker steroids present in NZA.
