Nitric Oxide Inhibitory Constituents from the Fruits of Amomum tsao-ko
10.20307/nps.2019.25.1.76
- Author:
Jun Gu KIM
1
;
Thi Phuong Linh LE
;
Hye Ryeong HONG
;
Jae Sang HAN
;
Jun Hwi KO
;
Seung Hyun LEE
;
Mi Kyeong LEE
;
Bang Yeon HWANG
Author Information
1. College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea. byhwang@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Amomum tsao-ko;
Zingiberaceae;
Nitric oxide;
Anti-inflammatory activity
- MeSH:
Amomum;
Chalcone;
Ether;
Fruit;
Macrophages;
Nitric Oxide;
Zingiberaceae
- From:Natural Product Sciences
2019;25(1):76-80
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bioactivity-guided fractionation of MeOH extract of the dried fruits of Amomum tsao-ko led to isolation of nine compounds (1 – 9). Their structures were elucidated by spectroscopic methods including extensive 1D and 2D-NMR, as alpinetin (1), naringenin-5-O-methyl ether (2), naringenin (3), hesperetin (4), 2′,4′,6′-trihydroxy-4-methoxy chalcone (5), tsaokoin (6), boesenbergin B (7), 4-hydroxyboesenbergin B (8), and tsaokoarylone (9). Of these, compound 8 was isolated from a natural source for the first time, which was previously reported as a synthetic product. The isolated compounds (1 – 9) were tested for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Among them, three chalcone derivatives (compounds 5, 7, and 8) and a diarylheptanoid (compound 9) exhibited significant inhibitory activity on the NO production with IC₅₀ values ranging from 10.9 to 22.5 µM.
