Isolation of Flavonoid Glycosides with Cholinesterase Inhibition Activity and Quantification from Stachys japonica
10.20307/nps.2018.24.4.259
- Author:
Agung NUGROHO
1
;
Jae Sue CHOI
;
Su Hui SEONG
;
Byong Min SONG
;
Kyoung Sik PARK
;
Hee Juhn PARK
Author Information
1. Department of Agro-industrial Technology, Lambung Mangkurat University, Banjarbaru 70714, Indonesia.
- Publication Type:Original Article
- Keywords:
Stachys japonica;
Lamiaceae;
isoscutellarein glycoside;
cholinesterase;
Alzheimer's disease
- MeSH:
Alzheimer Disease;
Butyrylcholinesterase;
Cholinesterases;
Chromatography, High Pressure Liquid;
Glycosides;
Lamiaceae;
Memory;
Plants;
Stachys
- From:Natural Product Sciences
2018;24(4):259-265
- CountryRepublic of Korea
- Language:English
-
Abstract:
The three flavone glycosides, 4′-O-methylisoscutellarein 7-O-(6‴-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (1), isoscutellarein 7-O-(6‴-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (3), and isoscutellarein 7-O-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (4) in addition to a flavonol glycoside, kaempferol 3-O-β-D-glucopyranoside (astragalin, 2), were isolated from Stachys japonica (Lamiaceae). In cholinesterase inhibition assay, compound 1 significantly inhibited aceylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (IC₅₀s, 39.94 µg/ml for AChE and 86.98 µg/ml for BChE). The content of isolated compounds were evaluated in this plant extract by HPLC analysis. Our experimental results suggest that the flavonoid glycosides of S. japonica could prevent the memory impairment of Alzheimer's disease.
