Efficient Isolation of Dihydrophaseic acid 3′-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography
10.20307/nps.2018.24.4.288
- Author:
Taewoong RHO
1
;
Kee Dong YOON
Author Information
1. College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon14662, Korea. kdyoon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Nelumbo nucifera;
Dihydrophaseic acid 3′-O-β-D-glucopyranoside;
High-performance countercurrent chromatography;
Isolation efficiency
- MeSH:
Chromatography;
Chromatography, Liquid;
Countercurrent Distribution;
Methods;
Nelumbo
- From:Natural Product Sciences
2018;24(4):288-292
- CountryRepublic of Korea
- Language:English
-
Abstract:
High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3′-O-β-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.
