Acer okamotoanum Inhibit the Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells
10.20307/nps.2018.24.3.148
- Author:
Soo Yeon CHOI
1
;
Ji Hyun KIM
;
Norman G QUILANTANG
;
Sanghyun LEE
;
Eun Ju CHO
Author Information
1. Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea. ejcho@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Acer okamotoanum;
C6 glial cell;
Inflammation;
Apoptosis;
Reactive oxygen species
- MeSH:
Acer;
Apoptosis;
Cell Survival;
Cyclooxygenase 2;
Hydrogen Peroxide;
Hydrogen;
Inflammation;
Neurodegenerative Diseases;
Neuroglia;
Neurons;
Neuroprotective Agents;
Nitric Oxide Synthase Type II;
Oxidative Stress;
Poly(ADP-ribose) Polymerases;
Reactive Oxygen Species
- From:Natural Product Sciences
2018;24(3):148-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chronic oxidative stress due to the accumulation of reactive oxygen species (ROS) in neuronal cells ultimately leads to neurodegenerative diseases. The use of natural therapies for the prevention of ROS-induced cell damage and for the treatment of neurodegenerative disorders has shown promising results. In this study, we evaluated the neuroprotective effects of the ethyl acetate (EtOAc) fraction of A. Okamotoanum against the hydrogen peroxide (H₂O₂)-induced oxidative stress in C6 glial cells. Results show that cell viability was decreased in cells incubated with H₂O₂, whereas the addition of EtOAc fraction treatments in such cells significantly increased viability. The EtOAc fraction showed the highest inhibitory activity against ROS production and it also decreased the expressions of inflammatory proteins including cyclooxygenase-2, inducible nitric oxide synthase and interleukin-1β. Furthermore, the EtOAc fraction inhibited apoptosis by regulating the protein expressions cleaved caspase
