Analysis of Essential oil, Quantification of Six Glycosides, and Nitric Oxide Synthase Inhibition Activity in Caryopteris incana
10.20307/nps.2018.24.3.181
- Author:
Agung NUGROHO
1
;
Sang Kook LEE
;
Donghwa KIM
;
Jae Sue CHOI
;
Kyoung Sik PARK
;
Byong Min SONG
;
Hee Juhn PARK
Author Information
1. Department of Agro-industrial Technology, Lambung Mangkurat University, Banjarbaru 70714, Indonesia.
- Publication Type:Original Article
- Keywords:
Caryopteris incana;
glycoside;
HPLC;
inducible nitric oxide synthase;
Verbenaceae
- MeSH:
Arthritis;
Chromatography, Gas;
Chromatography, High Pressure Liquid;
Cough;
Eczema;
Glycosides;
Macrophages;
Medicine, East Asian Traditional;
Nitric Oxide Synthase Type II;
Nitric Oxide Synthase;
Nitric Oxide;
Plants;
RAW 264.7 Cells;
Spectrum Analysis;
Verbenaceae
- From:Natural Product Sciences
2018;24(3):181-188
- CountryRepublic of Korea
- Language:English
-
Abstract:
Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental medicine. The two fractions (CHCl₃- and BuOH fractions) and the essential oil of the plant material were subjected to the inducible nitric oxide synthase (iNOS) assay. The IC₅₀ of the CHCl₃ fraction and the essential oil on LPS-induced macrophage RAW 264.7 cells were 16.4 µg/mL and 23.08 µg/mL, respectively. On gas chromatography (GC)-mass spectroscopy (MS) analysis, twenty-five components representing 85.5% amount of total essential oil were identified. On the chromatogram, three main substances, trans-pinocarveol, cis-citral, and pinocarvone, occupied 18.8%, 13.5% and 18.37% of total peak area. Furthermore, by HPLC-UV analysis, six compounds including one iridoid (8-O-acetylharpagide)- and five phenylethanoid glycosides (caryopteroside, acteoside, phlinoside A, 6-O-caffeoylphlinoside, and leucosceptoside A) isolated from the BuOH fraction were quantified. The content of six compounds were shown as the following order: caryopteroside (162.35 mg/g) > 8-O-acetylharpagide (93.28 mg/g) > 6-O-caffeoylphlinoside (28.15mg/g) > phlinoside (22.60mg/g) > leucosceptoside A (16.87 mg) > acteoside (7.05 mg/g).
