Simultaneous Determination of the Seven Phenylpropanoids in Xanthii Fructus Using a HPLC-PDA and LC-MS
10.20307/nps.2018.24.3.206
- Author:
Chang Seob SEO
1
;
Hyeun Kyoo SHIN
Author Information
1. Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. hkshin@kiom.re.kr
- Publication Type:Original Article
- Keywords:
Simultaneous;
Determination;
Xanthii Fructus;
Phenylpropanoids;
HPLC-PDA;
LC-MS
- MeSH:
Acetic Acid;
Arthritis, Rheumatoid;
Eczema;
Limit of Detection;
Methods;
Rhinitis;
Water
- From:Natural Product Sciences
2018;24(3):206-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
Xanthii Fructus has been traditionally used for the treatment of rhinitis, rheumatoid arthritis, and eczema. In this study, a high-performance liquid chromatography-photodiode array (HPLC-PDA) method was developed and then used for the simultaneous analysis of eight phenylpropanoids in Xanthii Fructus. The analytical column used for this separation was a SunFire™ C₁₈ column, maintained at 40℃. The mobile phase used was 1.0% acetic acid in distilled water and 1.0% acetic acid in acetonitrile with gradient elution. For identify of each component, the mass spectrometer (MS) was used a Waters triple quadrupole mass spectrometer requipped with electrospray ionization (ESI) source. The HPLC-PDA method showed good linearity: correlation coefficients were ≥ 0.9996. The limits of detection and quantification of the eight compounds were 0.02 – 0.04 and 0.06 – 0.14 µg/mL, respectively. The extraction recoveries ranged from 97.51 to 108.67%. The relative standard deviation values of intra- and inter-day precision were 0.06 – 1.55 and 0.09 – 1.68%, respectively. The validated HPLC-PDA method was applied to simultaneously analyse the amounts of eight phenlypropanoids in Xanthii Fructus.
