Ameliorative Effects of Ombuoside on Dopamine Biosynthesis in PC12 Cells
10.20307/nps.2018.24.2.99
- Author:
Uchralsaikhan DAVAASAMBUU
1
;
Keun Hong PARK
;
Hyun Jin PARK
;
Hyun Sook CHOI
;
Chong Kil LEE
;
Bang Yeon HWANG
;
Myung Koo LEE
Author Information
1. College of Pharmacy, Chungbuk National University, Cheongju 28160, Korea. myklee@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Ombuoside;
Dopamine biosynthesis;
TH;
CREB;
PC12 cells
- MeSH:
Animals;
Carrier Proteins;
Dopamine;
Levodopa;
PC12 Cells;
Phosphorylation;
Tyrosine 3-Monooxygenase
- From:Natural Product Sciences
2018;24(2):99-102
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the effects of ombuoside, a flavonol glycoside, on dopamine biosynthesis in PC12 cells. Ombuoside at concentrations of 1, 5, and 10 µM increased intracellular dopamine levels at 1 – 24 h. Ombuoside (1, 5, and 10 µM) also significantly increased the phosphorylation of tyrosine hydroxylase (TH) (Ser40) and cyclic AMP-response element binding protein (CREB) (Ser133) at 0.5 – 6 h. In addition, ombuoside (1, 5, and 10 µM) combined with L-DOPA (20, 100, and 200 µM) further increased intracellular dopamine levels for 24 h compared to L-DOPA alone. These results suggest that ombuoside regulates dopamine biosynthesis by modulating TH and CREB activation in PC12 cells.
