Simultaneous Determination of Four Compounds from Artemisia capillaris using High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD) and Their Quantitative Study in Artemisia Genus
10.20307/nps.2018.24.2.109
- Author:
Kiwon KO
1
;
In Kee HONG
;
Hyun Jong CHO
;
Young Ho KIM
;
Heejung YANG
Author Information
1. College of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea. yhk@cnu.ac.kr heejyang@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Artemisia capillaris;
Artemisia iwayomogi;
Artemisia princeps;
Artemisia argyi;
Scoparone;
HPLC-UV
- MeSH:
Artemisia;
Asian Continental Ancestry Group;
Chlorogenic Acid;
Coumarins;
Ethanol;
Fever;
Humans;
Jaundice;
Korea;
Liver;
Methods;
Plant Preparations
- From:Natural Product Sciences
2018;24(2):109-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.
