Pulegone Exhibits Anti-inflammatory Activities through the Regulation of NF-κB and Nrf-2 Signaling Pathways in LPS-stimulated RAW 264.7 cells
10.20307/nps.2018.24.1.28
- Author:
Anupom ROY
1
;
Hee Juhn PARK
;
Qudeer Ahmed ABDUL
;
Hyun Ah JUNG
;
Jae Sue CHOI
Author Information
1. Department of Food and Life Science, Pukyong National University, Busan 608-737, Republic of Korea. choijs@pknu.ac.kr
- Publication Type:Original Article
- Keywords:
Pulegone;
Anti-inflammation;
HO-1;
Nrf-2;
NF-κB
- MeSH:
Blotting, Western;
Down-Regulation;
Heme Oxygenase (Decyclizing);
Mitogen-Activated Protein Kinases;
NF-kappa B;
Nitric Oxide Synthase Type II;
Oils, Volatile;
Prostaglandin-Endoperoxide Synthases;
RAW 264.7 Cells;
Up-Regulation
- From:Natural Product Sciences
2018;24(1):28-35
- CountryRepublic of Korea
- Language:English
-
Abstract:
Pulegone is a naturally occurring organic compound obtained from essential oils from a variety of plants. The aim of this study was to investigate the anti-inflammatory effects through the inhibitory mechanism of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK) pathways and the activation of nuclear factor erythroid 2-related factor 2 (Nrf2)/ heme oxygenase (HO)-1 pathways in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Results revealed that pulegone significantly inhibited NO production as well as iNOS and COX-2 expressions. Meanwhile, western blot analysis showed that pulegone down-regulated LPS-induced NF-κB and MAPKs activation in RAW 264.7 cells. Furthermore, the selected compound suppressed LPS-induced intracellular ROS production in RAW 264.7 cells, while the expression of stress response gene, HO-1, and its transcriptional activator, Nrf-2 was upregulated upon pulegone treatment. Taking together, these findings provided that pulegone inhibited the LPS-induced expression of inflammatory mediators via the down-regulation iNOS, COX-2, NF-κB, and MAPKs signaling pathways as well as up-regulation of Nrf-2/HO-1 indicating that pulegone has a potential therapeutic and preventive application in various inflammatory diseases.
