Lignans with NADPH Oxidase 2 (NOX2)-inhibitory Activity from the Fruits of Schisandra chinensis
10.20307/nps.2018.24.1.59
- Author:
Jung Min PARK
1
;
Pisey PEL
;
Young Won CHIN
;
Moo Yeol LEE
Author Information
1. College of Pharmacy, Dongguk University, Goyang, Gyeonggi-do 10326, Republic of Korea. f2744@dongguk.edu, mlee@dongguk.edu
- Publication Type:Original Article
- Keywords:
NADPH oxidase 2 (NOX2);
NOX2 inhibitor;
Schisandra chinensis;
Gomisin T
- MeSH:
Chloroform;
Fruit;
Lignans;
Methanol;
NADP;
NADPH Oxidase;
Oxidoreductases;
Reactive Oxygen Species;
Schisandra;
Superoxides
- From:Natural Product Sciences
2018;24(1):59-65
- CountryRepublic of Korea
- Language:English
-
Abstract:
An isoform of NADPH oxidase (NOX), NOX2 is a superoxide-generating enzyme involved in diverse pathophysiological events. Although its potential as a therapeutic target has been validated, there is no clinically available inhibitor. Herein, NOX2-inhibitory activity was screened with the constituents isolated from Schisandra chinensis, which has been reported to have antioxidant and reactive oxygen species (ROS)-scavenging effects. Among the partitions prepared from crude methanolic extract, a chloroform-soluble partition showed the highest NOX2-inhibitory activity in PLB-985 cell-based NOX2 assay. A total of twenty nine compounds (1 – 29) were identified from the chloroform fraction, including two first isolated compounds; dimethyl-malate (25) and 2-(2-hydroxyacetyl) furan (27) from this plants. Of these constituents, two compounds (gomisin T, and pregomisin) exhibited an NOX2-inhibitory effect with the IC₅₀ of 9.4 ± 3.6, and 62.9 ± 11.3 µM, respectively. They are confirmed not to be nonspecific superoxide scavengers in a counter assay using a xanthine-xanthine oxidase system. These findings suggest the potential application of gomisin T (6) and other constituents of S. chinensis to inhibit NOX2.
