Isolation and identification of canine adenovirus type 2 from a naturally infected dog in Korea
10.14405/kjvr.2018.58.4.177
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Soon Seek YOON
;
Hyunkyoung LEE
;
In Soo CHO
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon 39660, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
canine adenovirus type 2;
dogs;
virus isolaltion
- MeSH:
Adenoviruses, Canine;
Animals;
Base Sequence;
Canada;
Dogs;
Erythrocytes;
Fluorescent Antibody Technique;
Genome;
Guinea Pigs;
Gyeonggi-do;
Hemagglutination;
Kidney;
Korea;
Madin Darby Canine Kidney Cells;
Microscopy, Electron;
Polymerase Chain Reaction;
Vaccines;
Vero Cells;
Vitis
- From:Korean Journal of Veterinary Research
2018;58(4):177-182
- CountryRepublic of Korea
- Language:English
-
Abstract:
Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached 10(6.5) 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.
