Comparison of hemostatic efficacy and cytotoxicity of three ferric subsulfate- and chitosan-based styptics in different formulations using a rat tail bleeding model
10.14405/kjvr.2018.58.3.119
- Author:
Jae Young BYUN
1
;
Soojung LEE
;
Jeong Ik LEE
;
Hun Young YOON
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea. yoonh@konkuk.ac.kr
- Publication Type:Original Article
- Keywords:
chitosan;
ferric subsulfate solution;
hemostasis;
hemostatics;
immunologic cytotoxicity
- MeSH:
Animals;
Cell Count;
Chitosan;
Cytotoxicity, Immunologic;
Fibroblasts;
Hemorrhage;
Hemostasis;
Hemostatics;
Lidocaine;
Mice;
Rats;
Tail;
Wounds and Injuries
- From:Korean Journal of Veterinary Research
2018;58(3):119-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to compare the hemostatic efficacy of three ferric subsulfate- and chitosan-based styptics as a powder and a gel containing ferric subsulfate and chitosan (FSC-PO and FSC-G, respectively) and a soaked pad containing ferric subsulfate and lidocaine (FSL-SP) using a rat tail bleeding model. The cytotoxicity of the styptics against L-929 mouse fibroblasts was also evaluated using a cell counting kit-8 assay. Four groups of 10 rats each were assigned to the three different styptics and a non-treated control groups. Rat tail tips were transected, after which styptics were applied with pressure. The wounds were observed for hemostasis for 3 min, then irrigated with saline to check for recurrent hemorrhage. L-929 mouse fibroblasts were exposed to extracts of the styptics (100 mg/mL) and their dilutions (1:10, 1:100, and 1:1,000). FSC-PO and FSC-G more effectively controlled initial hemorrhage than FSL-SP (p = 0.033). Additionally, FSC-PO and FSC-G more effectively maintained hemostasis than the control group (p = 0.02 and p < 0.01, respectively). However, all styptics showed enhanced cytotoxicity against L-929 cells in a dose-dependent manner. Therefore, although FSC-PO and FSC-G would be recommended to control hemorrhage, the benefits of styptics must be balanced against the clinical significance of their cytotoxicity.
