The effects of green tea (Camellia sinensis) flower extracton melanin synthesis in B16-F10 melanoma cells
10.14405/kjvr.2018.58.2.65
- Author:
Chanuri Yashara DISSANAYAKE
1
;
Hae Hee MOON
;
Kyeong Mi YANG
;
Younjae LEE
;
Chang Hoon HAN
Author Information
1. College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea. chhan@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
B16-F10 melanoma cells;
RNA sequencing analysis;
antioxidant effect;
green tea flower;
melanin synthesis inhibition;
tyrosinase activity inhibition
- MeSH:
Agaricales;
Antioxidants;
Flowers;
Gene Expression;
Hyperpigmentation;
Melanins;
Melanoma;
Monophenol Monooxygenase;
Sequence Analysis, RNA;
Tea
- From:Korean Journal of Veterinary Research
2018;58(2):65-72
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study observed the effects of a green tea (Camellia sinensis) flower extract (GTFE) on melanin synthesis in B16-F10 melanoma cells. GTFE exhibited antioxidant activity on 2,2-diphenyl-1-picrylhydrazyl and inhibited mushroom tyrosinase activity in a dose-dependent manner. Furthermore, GTFE significantly diminished α-melanocyte stimulating hormone (α-MSH) stimulated cellular melanin content and tyrosinase activity throughout the concentration range evaluated. Based on RNA sequencing analysis, differential gene expression patterns observed in α-MSH stimulated B16-F10 melanoma cells were normalized by the addition of GTFE. In particular, the expression levels of melanoregulin and tyrosinase genes which are key regulating genes in melanin synthesis were up-regulated by 3.5 and 3 fold respectively by α-MSH, and were normalized to control levels by the addition of GTFE. The results suggest that GTFE inhibits melanin synthesis in α-MSH stimulated B16-F10 melanoma cells by normalizing expression of genes that are essential for melanin synthesis. Overall, the results suggest that GTFE could be applied in the development of a whitening agent for the treatment of dermal hyperpigmentation.
