Molecular identification of coagulase-negative staphylococci by rpoB sequence typing
10.14405/kjvr.2018.58.1.51
- Author:
Won Jin SEONG
1
;
Danil KIM
;
Eun Kyung KIM
;
Dae Sung KO
;
Younghye RO
;
Jae Hong KIM
;
Hyuk Joon KWON
Author Information
1. Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. kwonhj01@snu.ac.kr
- Publication Type:Brief Communication
- Keywords:
16S rRNA gene sequencing;
bovine mastitis;
coagulase-negative staphylococci;
rpoB sequence typing
- MeSH:
Animals;
Bacterial Infections;
Cattle;
Diagnosis;
Epidemiology;
Female;
Genes, rRNA;
Mastitis, Bovine;
Methods
- From:Korean Journal of Veterinary Research
2018;58(1):51-55
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
