Studies on anticancer effect of momordin on oral carcinoma (KB) cells.
- Author:
Kyeong Seong SEO
1
;
Yeo Gab KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyung-Hee University.
- Publication Type:Original Article
- MeSH:
Apoptosis;
Bone Marrow;
Cell Cycle Checkpoints;
Cell Death;
DNA Fragmentation;
Flow Cytometry;
Humans;
Inhibitory Concentration 50;
KB Cells;
Mouth Neoplasms;
Plants, Medicinal
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2001;27(3):209-213
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on anti-oral cancer activities of momordin, which was medicinal plant extracts that was revealed anticancer activities, on KB cell(oral cancer cell). The results were as follow : 1. Momordin showed the excellent anti-oral cancer activity against KB cells. Obtained IC50 value of Momordin was 10.4 microgram/ml. 2. When KB cells were treated with Momordin, dose and time dependent DNA fragmentation of KB cells were observed. DNA fragmentation was initiated on three days at the concentration of 20 microgram/ml Momordin. 3. Flow cytometry showed dose-dependent apoptotic cell increase of KB cells on Momordin. 18.55% apoptotic cell were observed up to 72 hours at the concentration of 20 microgram/ml of Momordin 4. Momordin induced nonspecific apoptosis without specific cell cycle arrest. 5. Through MTT assay, DNA fragmentation assay and flow cytometric analysis. anticancer effect of Momordin against KB cell was induce of apoptotic cell death.
