A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay.
10.5145/KJCM.2011.14.1.36
- Author:
Chang Eun YOON
1
;
Young Joon HONG
;
Jin Kyung LEE
;
Yoon Hwan CHANG
;
Seok Il HONG
Author Information
1. Department of Laboratory Medicine, Korea Cancer Center Hospital, Seoul, Korea. hongsicp@kcch.re.kr
- Publication Type:Case Report
- Keywords:
Mycobacterium tuberculosis;
mpt64 gene;
Mutation
- MeSH:
Antibodies, Monoclonal;
Immunochromatography;
Mycobacterium;
Mycobacterium tuberculosis;
Nucleotides;
Polymerase Chain Reaction;
Proteins;
Sequence Analysis
- From:Korean Journal of Clinical Microbiology
2011;14(1):36-38
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Mycobacterium tuberculosis complex (MTBC) is discriminated from non-tuberculous mycobacteria (NTM) via an immunochromatographic assay (ICA) which is based on the reactions of monoclonal antibodies against MPT64, one of the predominant proteins excreted by MTBC. Recently, the authors of the present study discovered SD TB-negative Mycobacterium tuberculosis strains. In addition, sequence analysis of the mpt64 genes in these strains was performed and showed a deletion of 63 bp from nucleotides 196 to 258. In cases of MPT64-negative mycobacterium, the authors recommend performing TB PCR for correct diagnosis.
