Ethanol changes atpB gene expression and proton permeability in Streptococcus mutans
10.11149/jkaoh.2018.42.4.224
- Author:
Chul Min CHO
1
;
Yong Jin PARK
;
Sae A LEE
;
Jin Bom KIM
;
Jung Sook KANG
Author Information
1. Department of Oral Biochemistry & Molecular Biology, School of Dentistry, Pusan National University, Yangsan, Korea. jsokang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Ethanol;
atpB gene expression;
Proton permeability;
Streptococcus mutans
- MeSH:
Brain;
Cariostatic Agents;
Cell Membrane;
DNA, Complementary;
Electrodes;
Ethanol;
Fluorides;
Gene Expression;
Glass;
Heart;
Hydrogen-Ion Concentration;
Permeability;
Polymerase Chain Reaction;
Protons;
Real-Time Polymerase Chain Reaction;
RNA;
Streptococcus mutans;
Streptococcus;
Suspensions;
Toothpastes
- From:Journal of Korean Academy of Oral Health
2018;42(4):224-228
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: As a first step to study the anticaries effect of ethanol alone, we investigated the effects of ethanol on the expression levels of the atpB gene and proton permeability of Streptococcus mutans in suspension cultures. METHODS: S. mutans UA159 was grown in brain heart infusion medium at either pH 4.8 or 6.8. The total extracted RNA was reverse-transcribed into cDNA using a Superscript™ First-Strand Synthesis System. The resulting cDNA and negative controls were amplified by ABI PRISM 7700 real-time PCR system with SYBR Green PCR Master Mix. For proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then additional 0.5 M HCl was added to decrease the pH values by approximately 0.4 units. The subsequent increase in pH was monitored using a glass electrode. Ten percent (v/v) butanol was added to the suspensions at 80 min to disrupt the cell membrane. RESULTS: In a concentration-dependent manner, ethanol alone not only decreased the growth rate of S. mutans and the expression of the atpB gene but also increased the proton permeability at both pH 4.8 and 6.8. CONCLUSIONS: These findings suggest that ethanol has the potential for an anticaries ingredient. We believe that ethanol may be used together with fluoride and/or other cariostatic agents in order to develop better anticaries toothpastes and/or mouthrinses.
