Antioxidant and growth inhibitory activities of Mesembryanthemum crystallinum L. in HCT116 human colon cancer cells
10.4163/jnh.2019.52.2.157
- Author:
Jin A SEO
1
;
Jihyeung JU
Author Information
1. Department of Food and Nutrition, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. jujih@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
ice plant;
reactive oxygen species;
cancer cell growth;
apoptosis;
cell cycle
- MeSH:
Apoptosis;
Cell Cycle;
Cell Cycle Checkpoints;
Colon;
Colonic Neoplasms;
Ethanol;
Gallic Acid;
HCT116 Cells;
Humans;
Mesembryanthemum;
Reactive Oxygen Species;
Water
- From:Journal of Nutrition and Health
2019;52(2):157-167
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study examined the antioxidant and cancer cell growth inhibitory activities of an ethanol extract and different solvent fractions of Mesembryanthemum crystallinum L. (ice plant). METHODS: The ice plant was freeze-dried, extracted with 99.9% ethanol, and then fractionated with hexane, ethyl acetate, butanol, and water. The total polyphenol content (TPC), total carotenoid content (TCC), 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity (RSA), and ferric reducing antioxidant power (FRAP) were measured. Assays using 2′,7′-dichlorofluorescin-diacetate and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide were performed to measure the intracellular reactive oxygen species (ROS) and cell growth, respectively. Annexin V/propidium iodide staining and cell cycle analysis were performed for the detection of apoptosis and cell cycle arrest. RESULTS: TPC, TCC, RSA, and FRAP of the ethanol extract (EE) were 3.7 mg gallic acid equivalent/g, 13.2 µg/g, 21.0% (at a concentration of 5 mg/mL), and 21.0% (at a concentration of 5 mg/mL), respectively. Among the different solvent fractions, the butanol fraction (BF) showed the highest TPC (5.4 mg gallic acid equivalent/g), TCC (86.6 µg/g), RSA (34.9% at 5 mg/mL), and FRAP (80.8% at 5 mg/mL). Treatment of HCT116 human colon cancer cells with EE and BF at concentrations of 250 and 500 µg/mL reduced the levels of intracellular ROS. Concomitantly, EE and BF resulted in the dose-dependent inhibition of cell growth (at the concentrations of 125, 250, and 500 µg/mL for 24 ~ 48 h) and the induction of apoptosis (at the concentrations of 250 and 500 µg/mL for 48 h) in HCT116 cells. An increased G2/M cell population was also found in the BF-treated cells. CONCLUSION: These results suggest that ice plant possesses antioxidant and growth inhibitory activities in colon cancer cells.
