Anti-melanogenic effects of Hordeum vulgare L. barely sprout extract in murine B16F10 melanoma cells
10.4163/jnh.2019.52.2.168
- Author:
Jeong Hwa CHOI
1
;
Jong Gi JUNG
;
Jung Eun KIM
;
Mi Ae BANG
Author Information
1. Department of Food Science and Nutrition, Keimyung University, Daegu 42601, Korea.
- Publication Type:Original Article
- Keywords:
Hordeum vulgare L;
melanocyte;
tyrosinase
- MeSH:
Arbutin;
Chromatography, High Pressure Liquid;
Herbal Medicine;
Hordeum;
In Vitro Techniques;
Insurance Benefits;
Melanins;
Melanocytes;
Melanoma;
Microphthalmia-Associated Transcription Factor;
Monophenol Monooxygenase;
Polyphenols;
Therapeutic Uses;
Vanillic Acid;
Water
- From:Journal of Nutrition and Health
2019;52(2):168-175
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Barely sprout is a well-known oriental herbal medicine with a wide range of health benefits. Recent studies have provided scientific evidence of its therapeutic effects with expanded application. This study investigated anti-melanogenic effect of barley sprout water extract (BSE) in murine melanocyte B16F10. METHODS: Various concentrations (0, 50, 125, and 250 µg/mL) of BSE and arbutin (150 ppm) were applied to B16F10 stimulated with or without alpha-melanocyte stimulating hormone (100 nM) for 72 hours. The whitening potency of BSE was determined altered cellular melanin contents. Activity and expression of tyrosinase and microphthalmia-associated transcription factor (MITF) were also assayed. RESULTS: Experimental results revealed that treatment with BSE reduced cellular melanin production by approximately 40% compared to the control. Molecular findings supported that suppressed activity and expression of tyrosinase and MITF proteins by BSE were associated with declined cellular melanogenesis. Furthermore, anti-melanogenic effect of BSE (250 µg/mL) was similar to that of arbutin, a commonly used whitening agent. Lastly, polyphenols including p-coumaric, ferulic, and vanillic acids were identified in BSE using HPLC analyses. They might be potential active ingredients showing such melanogenesis-reducing effect. CONCLUSION: BSE was evident to possess favorable anti-melanogenic potency in an in vitro model. As a natural food sourced material, BSE could be an effective depigmentation agent with potential application in pharmaceutical and cosmetic industries.
