Expression and Localization of Keap1 During Amelogenesis in the Developing Molar Germ of Rats
10.11620/IJOB.2018.43.4.177
- Author:
Sun Hun KIM
1
;
Yong Ouk YOU
;
Hyun Mi KO
;
Hyun Jin KIM
Author Information
1. Dental Science Research Institute, Chonnam National University, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Keap1;
ameloblasts;
amelogenesis;
tooth development;
enamel formation
- MeSH:
Ameloblasts;
Amelogenesis;
Amelogenin;
Animals;
Blotting, Western;
Dental Enamel;
Fluorescent Antibody Technique;
Molar;
Polymerase Chain Reaction;
Rats;
Reverse Transcription;
RNA, Messenger
- From:International Journal of Oral Biology
2018;43(4):177-183
- CountryRepublic of Korea
- Language:English
-
Abstract:
The objective of this study was to examine the expression pattern of Kelch-like ECH-associated protein 1 (Keap1) in the maxillary 2(nd) molar germs of rats. We used the maxillary 2(nd) molar germs in rats' pup at postnatal day 3 (bell stage), 6 (crown formation stage) and 9 (root formation stage). The investigation on mRNA and protein levels were done using reverse transcription - polymerase chain reaction and western blot. Localization of Keap 1 in the maxillary 2(nd) molar germs were revealed through immunofluorescence staining. Keap1 from the maxillary 2nd molar germs were mostly manifested on postnatal day 3 and dramatically decreased on postnatal day 6 and 9 at mRNA and protein levels, while amelogenin and ameloblastin increased during the development of maxillary 2nd molar germs. During immunofluorescence analysis, the strong immunoreactivity against Keap1 was detected in the apical side of ameloblasts at the presecretory and secretory stages. However, Keap1 expression was hardly observed in the ameloblasts at the maturation stage. These results shows that Keap1 is strongly expressed in the presecretory and secretory ameloblasts of amelogenesis, and suggest that Keap1 may be a crucial molecule for the regulatory mechanisms tasked with the formation of enamel layer.
