Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells
10.11620/IJOB.2018.43.2.077
- Author:
Eun Jin SEO
1
;
Jae Kyung PARK
;
Hoim JEONG
;
Jung Sook KANG
;
Hyung Ryong KIM
;
Il Ho JANG
Author Information
1. Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry, Yangsan 50612, Gyeongsangnam-do, Republic of Korea. ilho.jang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
dental pulp stem cell;
organoid culture;
endothelial cell;
CD31
- MeSH:
Dental Pulp;
Dentistry;
Endothelial Cells;
Extracellular Matrix;
Flow Cytometry;
Humans;
Immunohistochemistry;
Mesenchymal Stromal Cells;
Molar;
Organoids;
Stem Cells
- From:International Journal of Oral Biology
2018;43(2):77-82
- CountryRepublic of Korea
- Language:English
-
Abstract:
The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.
