Changes in Oral Microbiota in Patients Receiving Radical Concurrent Chemoradiotherapy for The Head and Neck Squamous Cell Carcinoma
10.11620/IJOB.2018.43.1.013
- Author:
Jin Ho KIM
1
;
Yoon Hee CHOI
;
Soo Youn AN
;
Hee Young SON
;
Chulwon CHOI
;
Seyeon KIM
;
Jin CHUNG
;
Hee Sam NA
Author Information
1. Department of Radiation Oncology, Seoul National University Hospital, Seoul 03080, South Korea.
- Publication Type:Original Article
- Keywords:
Pyrosequencing;
Oral microbiota;
Head and neck squamous cell carcinoma;
Concurrent chemoradiotherapy
- MeSH:
Anti-Bacterial Agents;
Bacteroides;
Carcinoma, Squamous Cell;
Chemoradiotherapy;
Citrus sinensis;
Drug Therapy;
Epithelial Cells;
Firmicutes;
Fusobacteria;
Genes, rRNA;
Head and Neck Neoplasms;
Head;
Humans;
Microbiota;
Neck;
Oral Hygiene;
Proteobacteria;
Radiotherapy
- From:International Journal of Oral Biology
2018;43(1):13-21
- CountryRepublic of Korea
- Language:English
-
Abstract:
Radiotherapy (RT) is a mainstay in the treatment of head and neck squamous cell carcinoma (HNSCC). For locally advanced HCSCC, concurrent chemoradiotherapy (CCRT) benefits HCSCC patients in terms of better survival and loco-regional control. In this study, we evaluated changes in oral microbiota in patients, who received CCRT for head and neck cancer. Oral rinsed samples were weekly collected before and during CCRT and at 4 weeks following treatment from HNSCC patients, who had received 70 Gy of radiation delivered to the primary sites for over 7 weeks and concurrent chemotherapy. Oral microbiota changes in three patients were analyzed by next-generation sequencing using 16S rRNA 454 pyrosequencing. On an average, 15,000 partial 16S rRNA gene sequences were obtained from each sample. All sequences fell into 11 different bacterial phyla. During early CCRT, the microbial diversity gradually decreased. In a patient, who did not receive any antibiotics during the CCRT, Firmicutes and Proteobacteria were the most abundant phylum. During the early CCRT, proteobacteria gradually decreased while Firmicutes increased. During the late CCRT, firmicutes gradually decreased while Bacteroides and Fusobacteria increased. In all the patients, yellow complex showed a gradual decrease, while orange and red complex showed a gradual increase during the CCRT. At 4 weeks after CCRT, the recovery of oral microbiota diversity was limited. During CCRT, there was a gradual increase in major periodontopathogens in association with the deterioration of the oral hygiene. Henceforth, it is proposed that understanding oral microbiota shift should provide better information for the development of effective oral care programs for patients receiving CCRT for HNSCC.
