Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease
- Author:
Tae Geun GWEON
1
;
Jong Hyung PARK
;
Byung Wook KIM
;
Yang Kyu CHOI
;
Joon Sung KIM
;
Sung Min PARK
;
Chang Whan KIM
;
Hyung Gil KIM
;
Jun Won CHUNG
;
Author Information
- Publication Type:Original Article
- Keywords: Gastroesophageal reflux; Rebamipide; Tight junction protein
- MeSH: Animals; Blotting, Western; Claudin-1; Claudin-3; Gastroesophageal Reflux; Leukocytes; Models, Animal; Mucous Membrane; Proton Pump Inhibitors; Proton Pumps; Protons; Rats; Stomach; Tight Junction Proteins; Tight Junctions
- From:Gut and Liver 2018;12(1):46-50
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: The aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD). METHODS: GERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups. An additional dose of rebamipide (100 mg/kg) was administered orally to the PPI+R group. Mucosal erosions, epithelial thickness, and leukocyte infiltration into the esophageal mucosa were measured in isolated esophagi 14 days after the procedure. A Western blot analysis was conducted to measure the expression of claudin-1, -3, and -4. RESULTS: The mean surface area of mucosal erosions, epithelial thickness, and leukocyte infiltration were lower in the PPI group and the PPI+R group than in the control group. Western blot analysis revealed that the expression of claudin-3 and -4 was significantly higher in the PPI+R group than in the control group. CONCLUSIONS: Rebamipide may exert an additive effect in combination with PPI to modify the tight junction proteins of the esophageal mucosa in a rat model of GERD. This treatment might be associated with the relief of GERD symptoms.
