MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression
- Author:
Tae Won HA
1
;
Hyun Soo KANG
;
Tae Hee KIM
;
Ji Hyun KWON
;
Hyun Kyu KIM
;
Aeli RYU
;
Hyeji JEON
;
Jaeseok HAN
;
Hal E BROXMEYER
;
Yongsung HWANG
;
Yun Kyung LEE
;
Man Ryul LEE
Author Information
- Publication Type:Original Article
- Keywords: microRNA; CXCR4; Cord blood; Cell migration
- MeSH: Cell Line; Cell Movement; Chemotaxis; Fetal Blood; Hematopoietic Stem Cells; MicroRNAs; Stromal Cells
- From:International Journal of Stem Cells 2018;11(2):187-195
- CountryRepublic of Korea
- Language:English
- Abstract: Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34⁺ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34⁺ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34⁺ cells.
