Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

Author: Min Ah YU ¹ ; Hye Won JEONG ; Su Jin PARK ; Young Il KIM ; Hyeok Il KWON ; Eun Ha KIM ; Young Jae SI ; Kwang Min YU ; Norbert John ROBLES ; Hae Jung HAN ; Young Ki CHOI
Author Information

1. Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine, Cheongju, Korea. choiki55@chungbuk.ac.kr
Publication Type:Brief Communication
Keywords: Severe fever with thrombocytopenia syndrome virus; Enzyme-linked immunosorbent assay; NP protein; Gn protein
MeSH: Diagnosis; Enzyme-Linked Immunosorbent Assay; Fever; Genotype; Nucleocapsid Proteins; Sensitivity and Specificity; Thrombocytopenia
From:Clinical and Experimental Vaccine Research 2018;7(1):82-86
CountryRepublic of Korea
Language:English
Abstract: To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.