Protective effect of enzymatic hydrolysates from highbush blueberry (Vaccinium corymbosum L.) against hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast cell line.
- Author:
Mahinda SENEVIRATHNE
1
;
Soo Hyun KIM
;
You Jin JEON
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Blueberry; enzymatic hydrolysates; water soluble compounds; H2O2 induced damage; ROS
- MeSH: Animals; Asian Continental Ancestry Group; Blueberry Plant; Cell Line; Cell Survival; Cricetinae; Cricetulus; DNA Damage; Endopeptidases; Fibroblasts; Humans; Hydrogen; Hydrolysis; Lipid Peroxidation; Lung; Metalloendopeptidases; Peptide Hydrolases; Phenol; Subtilisins
- From:Nutrition Research and Practice 2010;4(3):183-190
- CountryRepublic of Korea
- Language:English
- Abstract: Blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) and proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex) to obtain water soluble compounds, and their protective effect was investigated against H2O2-induced damage in Chinese hamster lung fibroblast cell line (V79-4) via various published methods. Both AMG and Alcalase hydrolysates showed higher total phenolic content as well as higher cell viability and ROS scavenging activities, and hence, selected for further antioxidant assays. Both AMG and Alcalase hydrolysates also showed higher protective effects against lipid peroxidation, DNA damage and apoptotic body formation in a dose-dependent fashion. Thus, the results indicated that water soluble compounds obtained by enzymatic hydrolysis of blueberry possess good antioxidant activity against H2O2-induced cell damage in vitro.
