Neuroprotective effects of consuming bovine colostrum after focal brain ischemia/reperfusion injury in rat model.
- Author:
Han Sung CHOI
1
;
Young Gwan KO
;
Jong Seok LEE
;
Oh Young KWON
;
Sun Kyu KIM
;
Chul CHEONG
;
Ki Hyo JANG
;
Soon Ah KANG
Author Information
- Publication Type:Original Article
- Keywords: Focal brain ischemia/reperfusion; bovine colostrum; cytokines; neuroprotection
- MeSH: Animals; Brain; Brain Injuries; Colostrum; Cytokines; Eating; Interleukin-6; Milk; Neuroprotective Agents; Rats; Rats, Sprague-Dawley; Reperfusion; Salicylamides; Tumor Necrosis Factor-alpha
- From:Nutrition Research and Practice 2010;4(3):196-202
- CountryRepublic of Korea
- Language:English
- Abstract: To investigate the neuroprotective effects of bovine colostrums (BC), we evaluate the ability of consuming BC after focal brain ischemia/reperfusion injury rat model to reduce serum cytokine levels and infarct volume, and improve neurological outcome. Sprague-Dawley rats were randomly divided into 4 groups; one sham operation and three experimental groups. In the experimental groups, MCA occlusion (2 h) and subsequent reperfusion (O/R) were induced with regional cerebral blood flow monitoring. One hour after MCAO/R and once daily during the experiment, the experimental group received BC while the other groups received 0.9% saline or low fat milk (LFM) orally. Seven days later, serum pro-inflammatory cytokine (IL-1beta, IL-6, and TNF-alpha) and anti-inflammatory cytokine (IL-10) levels were assessed. Also, the infarct volume was assessed by using a computerized image analysis system. Behavioral function was also assessed using a modified neurologic severity score and corner turn test during the experiment. Rats receiving BC after focal brain I/R showed a significant reduction (-26%/-22%) in infarct volume compared to LFM/saline rats, respectively (P < 0.05). Serum IL-1beta, IL-6, and TNF-alpha levels were decreased significantly in rats receiving BC compared to LFM/saline rats (P < 0.05). In behavioral tests, daily BC intake showed consistent and significant improvement of neurological deficits for 7 days after MCAO/R. BC ingestion after focal brain ischemia/reperfusion injury may prevent brain injury by reducing serum pro-inflammatory cytokine levels and brain infarct volume in a rat model.
