Intranasal Treatment With 1, 25-Dihydroxyvitamin D3 Alleviates Allergic Rhinitis Symptoms in a Mouse Model
10.4168/aair.2019.11.2.267
- Author:
Sung Woo CHO
1
;
Yu Lian ZHANG
;
Young Kyung KO
;
Jae Min SHIN
;
Jun Ho LEE
;
Chae Seo RHEE
;
Dong Young KIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.
- Publication Type:Original Article
- Keywords:
Animal model;
allergic rhinitis;
dendritic cell;
intranasal administration;
vitamin D;
antiallergic agents
- MeSH:
Administration, Intranasal;
Animals;
Anti-Allergic Agents;
Calcitriol;
Cell Proliferation;
Dendritic Cells;
Eosinophils;
Flow Cytometry;
Immunoglobulin E;
Immunoglobulin G;
Immunoglobulins;
Inflammation;
Interleukin-10;
Interleukin-13;
Interleukin-4;
Interleukin-5;
Lymph Nodes;
Lymphocytes;
Major Histocompatibility Complex;
Mice;
Models, Animal;
Ovalbumin;
Ovum;
Rhinitis, Allergic;
RNA, Messenger;
Vitamin D
- From:Allergy, Asthma & Immunology Research
2019;11(2):267-279
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Vitamin D is a potent immunomodulator. However, its role in the pathogenesis of allergic rhinitis is unclear. METHODS: The aim of this study was to evaluate the antiallergic effect of intranasally applied vitamin D in an allergic rhinitis mouse model. BALB/c mice were intraperitoneally sensitized with ovalbumin (OVA) and alum before they were intranasally challenged with OVA. Then, they were intranasally administered 1, 25-dihydroxyvitamin D3 (0.02 μg) or solvent. Allergic symptom scores, eosinophil infiltration, cytokine mRNA levels (interleukin [IL]-4, IL-5, IL-10, IL-13 and interferon-γ) in the nasal tissue, and serum total immunoglobulin E (IgE) and OVA-specific IgE, IgG1, and IgG2a were analyzed and compared with negative and positive control groups. Cervical lymph nodes (LNs) were harvested for flow cytometry analysis and cell proliferation assay. RESULTS: In the treatment group, allergic symptom scores, eosinophil infiltration, and mRNA levels of IL-4 and IL-13 were significantly lower in the nasal tissue than in the positive control group. The IL-5 mRNA level, serum total IgE, and OVA-specific IgE and IgG1 levels decreased in the treatment group; however, the difference was not significant. In the cervical LNs, CD86 expression had been down-regulated in CD11c+major histocompatibility complex II-high (MHCIIhigh) in the treatment group. Additionally, IL-4 secretion in the lymphocyte culture from cervical LNs significantly decreased. CONCLUSIONS: The results confirm the antiallergic effect of intranasal 1,25-dihydroxyvitamin D3. It decreases CD 86 expression among CD11c+MHCIIhigh cells and T-helper type 2-mediated inflammation in the cervical LNs. Therefore, topically applied 1,25-dihydroxyvitamin D3 can be a future therapeutic agent for allergic rhinitis.
