Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
- Author:
Aysegul BATIOGLU-KARAALTIN
1
;
Ercüment OVALI
;
Mehmet V KARAALTIN
;
Murat YENER
;
Mehmet YILMAZ
;
Fatma EYÜPOĞLU
;
Yetkin Zeki YILMAZ
;
Erol Rüştü BOZKURT
;
Necdet DEMIR
;
Esma KONUK
;
Ergun Süreyya BOZDAĞ
;
Ozgür YIĞIT
;
Harun CANSIZ
Author Information
- Publication Type:Original Article
- Keywords: Trachea; Scaffold; Freeze Drying; Tissue Engineering; Deoxycholic Acid
- MeSH: Deoxycholic Acid; DNA; Freeze Drying; Glucose; Lactic Acid; Rabbits; Tissue Engineering; Trachea
- From:Clinical and Experimental Otorhinolaryngology 2019;12(1):86-94
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. METHODS: Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. RESULTS: DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). CONCLUSION: Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.
