Enumeration of CD34-positive Stem Cells Using the ADAMII Image-based Fluorescence Cell Counter
10.3343/alm.2019.39.4.388
- Author:
Haein YU
1
;
Jaeeun YOO
;
Jung Sil HWANG
;
Mikyung KIM
;
Kyung Hee BAE
;
Dong Wook JEKARL
;
Jong Hyun OH
;
Ji Yeon LEE
;
Sunmi HAN
;
Chanil CHUNG
;
Myungshin KIM
;
Yonggoo KIM
Author Information
1. Laboratory Development and Evaluation Center, College of Medicine, The Catholic University of Korea, Seoul, Korea. yonggoo@catholic.ac.kr, microkim@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
CD34;
Enumeration;
Image-based fluorescence cell counter;
Flow cytometry;
Performance
- MeSH:
Blood Component Removal;
Cell Count;
Flow Cytometry;
Fluorescence;
Hematopoietic Stem Cell Transplantation;
Humans;
Leukapheresis;
Seoul;
Stem Cells;
Tissue Donors
- From:Annals of Laboratory Medicine
2019;39(4):388-395
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: It is very important to accurately enumerate CD34-positive (CD34+) cells for successful hematopoietic stem cell transplantation (HSCT). We evaluated the ability of the newly developed image based-immunofluorescence cell counter ADAMII (NanoEntek, Seoul, Korea) to enumerate CD34+ cells, which was improved through simultaneous CD45 analysis. METHODS: We enumerated CD34+ cells with ADAMII using 19 peripheral blood (PB) and 91 leukapheresis samples from HSCT donors. Analytical performance, including precision and linearity, was analyzed, and sample stability during storage was evaluated. Viable CD34+ cell count (vCD34) and viable CD45+ cell count (vCD45) and the percentage of viable CD34+ cells among viable CD45+ cells (CD34/CD45) as measured by ADAMII were compared with the corresponding values from two flow cytometry assays, using regression analysis. RESULTS: ADAMII demonstrated acceptable precision, as CV values of vCD34 from six samples with different counts were all < 10% (range: 3.49–9.51%). CV values of the vCD45 and CD34/45 ranged from 4.03% to 9.67% and from 2.48% to 10.07%, respectively. The linearity of vCD34 showed an excellent R 2 value (0.99) when analyzed using the intended count and flow cytometry data. The ADAMII and two flow cytometry-based assays generated very similar data for the PB and leukapheresis samples. CONCLUSIONS: ADAMII demonstrated excellent performance for use as a routine clinical assay in terms of CD34+ cell enumeration from PB and leukapheresis samples. Moreover, it could be used as a point-of-care-test for determining mobilization time and predicting an adequate apheresis stem cell product.
