Mosquitoes, midges and related arboviruses in southeast Sichuan province.
10.3760/cma.j.issn.0254-6450.2018.10.017
- Author:
S SONG
1
,
2
;
S H FU
3
;
X X ZHOU
4
;
J K ZHANG
4
;
W LI
4
;
L J LIU
4
;
J S LI
5
;
J WANG
6
;
Y LIN
7
;
X L LI
3
;
Y HE
3
;
W W LEI
3
;
H Y WANG
3
;
B WANG
8
;
X Q LU
8
;
G D LIANG
3
Author Information
1. Qingdao University, Qingdao 266071, China
2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
3. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
4. Sichuan Province Center for Disease Control and Prevention, Chengdu 610041, China.
5. Luzhou Municipal Center for Disease Control and Prevention, Luzhou 646000, China.
6. Hejiang County Center for Disease Control and Prevention, Hejiang 646299, China.
7. Nanxi District Center for Disease Control and Prevention, Yibin 644199, China.
8. Qingdao University, Qingdao 266071, China.
- Publication Type:Journal Article
- Keywords:
Akabane virus;
Japanese encephalitis virus;
Midge;
Mosquito
- MeSH:
Animals;
Arboviruses;
Culicidae;
Encephalitis Virus, Japanese/isolation & purification*;
Encephalitis, Japanese/diagnosis*;
Genes, Viral;
Nucleic Acid Amplification Techniques;
Phylogeny;
Polymerase Chain Reaction
- From:
Chinese Journal of Epidemiology
2018;39(10):1381-1386
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the distribution patterns of mosquitoes, midges and related arboviruses in Sichuan province. Methods: Blood-sucking insects were collected from houses and pens, using the ultraviolet lights. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. All samples were incubated with BHK-21 and C6/36 cells for virus isolation and then detected for their viral genes. Sequences of the virus were identified and analyzed by molecular biological software, such as BioEdit 7.0.5.3, MEGA 6.0. Results: In total, 17 019 mosquitoes from 3 genera and 4 species and 12 700 midges were collected from the southeast regions of Sichuan province in 2016 and 2017. Among them, 79.4% (13 519/17 019) belonged to Culex tritaeniorhynchus with 11.1% (1 897/17 019) as Armigeres subalbatus, 5.5% (930/17 019) were Anopheles sinensis and 4.0% (673/17 019) were Anopheles sinensis 3 virus strains that isolated from Culex tritaeniorhynchus were identified as typeⅠ Japanese encephalitis virus. Seven pools of mosquitoes isolated from Hejiang county were identified Japanese encephalitis virus gene positive through PCR amplification. With 4 pool midges were detected positive for Akabane virus through PCR gene amplification while midges samples didn't have virus isolates. Conclusions: Culex tritaeniorhynchus appeared the predominant species in the southeast regions of Sichuan. Japanese encephalitis virus transmitted by mosquitoes and Akabane virus by midges were prevalent in southeast Sichuan province.
