Study on the super-antigen genes of group A Streptococcus pyogenes strains isolated from patients with scarlet fever and pharyngeal infection, in Beijing, 2015-2017.
10.3760/cma.j.issn.0254-6450.2018.10.016
- VernacularTitle:北京市2015-2017年猩红热及咽部感染患者A组链球菌超抗原基因特征研究
- Author:
C N MA
1
;
X M PENG
;
S S WU
;
D T ZHANG
;
J C ZHAO
;
G L LU
;
Y PAN
;
S J CUI
;
Y M LIU
;
W X SHI
;
M ZHANG
;
Q Y WANG
;
P YANG
Author Information
1. Beijing Municipal Center for Disease Control and Prevention, Beijing Research Center for Preventive Medicine, Beijing 100013, China.
- Publication Type:Journal Article
- Keywords:
Group A Streptococcus pyogenes;
Pharyngeal infection;
Scarlet fever;
Superantigen;
emm genes
- MeSH:
Antigens, Bacterial/genetics*;
Bacterial Outer Membrane Proteins;
Bacterial Proteins;
Beijing/epidemiology*;
China/epidemiology*;
Exotoxins;
Female;
Humans;
Membrane Proteins;
Pharyngitis/microbiology*;
Pharynx/microbiology*;
Pregnancy;
Pregnancy Complications, Infectious/microbiology*;
Real-Time Polymerase Chain Reaction;
Scarlet Fever/microbiology*;
Streptococcal Infections;
Streptococcus pyogenes/isolation & purification*;
Superantigens/genetics*
- From:
Chinese Journal of Epidemiology
2018;39(10):1375-1380
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the characteristics of super-antigen (SAg) of group A Streptococcus pyogenes (GAS), isolated from patients with scarlet fever or pharyngeal infections in Beijing between 2015-2017. Methods: Throat swab specimens from patients with scarlet fever or pharyngeal infections were collected and tested for GAS. Eleven currently known SAg genes including SpeA, speC, speG, speH, speI, speJ, speK, speL, speM, smeZ and ssa were tested by real-time PCR while M protein genes (emm genes) were amplified and sequenced by PCR. Results: A total of 377 GAS were isolated from 6 801 throat swab specimens, with the positive rate as 5.5%. There were obvious changes noticed among speC, speG, speH and speK in three years. A total of 45 SAg genes profiles were observed, according to the SAgs inclusion. There were significant differences appeared in the frequencies among two of the highest SAg genes profiles between emm1 and emm12 strains (χ(2)=38.196, P<0.001; χ(2)=72.310, P<0.001). There also appeared significant differences in the frequencies of speA, speH, speI and speJ between emm1 and emm12 strains (χ(2)=146.154, P<0.001; χ(2)=52.31, P<0.001; χ(2)=58.43, P<0.001; χ(2)=144.70, P<0.001). Conclusions: Obvious changes were noticed among SAg genes including speC, speG, speH and speK from patients with scarlet fever or pharyngeal infections in Beijing between 2015-2017. SAg genes including speA, speH, speI and speJ appeared to be associated with the emm 1 and emm 12 strains. More kinds of SAg genes profiles were isolated form GAS but with no significant differences seen in the main SAg genes profiles, during the epidemic period.
