Analysis on influencing factors that leading to nonspecific responses to indeterminate results of HIV antibodies.
10.3760/cma.j.issn.0254-6450.2018.09.021
- Author:
S L WU
1
,
2
,
3
;
M GAO
4
;
J ZHENG
5
;
P P YAN
1
,
3
;
Y S YAN
1
,
2
,
3
Author Information
1. Institute for AIDS/STD Control and Prevention, Fujian Provincial Center for Disease Control and Prevention, Fuzhou 350001, China
2. School of Public Health, Fujian Medical University, Fuzhou 350004, China
3. Fujian Province Key Laboratory of Zoonosis Research, Fuzhou 350001, China.
4. School of Public Health, Fujian Medical University, Fuzhou 350004, China.
5. Institute for AIDS/STD Control and Prevention, Fujian Provincial Center for Disease Control and Prevention, Fuzhou 350001, China.
- Publication Type:Journal Article
- Keywords:
HIV antibody;
Indeterminate;
Influencing factor;
Nonspecific responses
- MeSH:
Adult;
Blotting, Western/methods*;
Case-Control Studies;
China/epidemiology*;
Enzyme-Linked Immunosorbent Assay/methods*;
HIV Antibodies/isolation & purification*;
HIV Infections/epidemiology*;
HIV-1/immunology*;
Humans;
Middle Aged;
Predictive Value of Tests;
Sensitivity and Specificity
- From:
Chinese Journal of Epidemiology
2018;39(9):1255-1260
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the influencing factors that leading to nonspecific responses to indeterminate HIV antibody tests, to provide scientific evidence for the differential diagnosis of HIV infection and control strategy. Methods: A case control study was conducted. The samples of HIV antibody indeterminate in confirmed Western blot (WB) tests, but were negative in HIV nucleic acid tests, were collected as HIV antibody indeterminate group from WB results of HIV confirmatory laboratories of Fujian province in 2015-2016. The general population matched group with HIV antibody screening negative samples and WB negative matched group with WB negative samples were selected as the two compared groups by matching gender and age from HIV antibody screening in Fujian province in the same period. Blood concentrations of hepatitis B surface antigen (HBsAg), anti-hepatitis C virus (HCV) antibody, anti-treponema pallidum (TP) antibody, antinuclear antibody (ANA), anti-human T-cell leukemia virus (HTLV) antibody, and alpha-fetoprotein (AFP) were detected by using enzyme-linked immunosorbent assay (ELISA). χ(2) test and multivariate non-conditional logistic regression analysis were performed to identify the influencing factors that leading to nonspecific responses, to indeterminate HIV antibody tests. Results: A total of 13 WB band patterns were observed in 110 HIV antibody indeterminate samples, in which a single p24 band (58.18%, 64/110), a single gp160 band (17.27%, 19/110) and a single p17 band (7.27%, 8/110) were the three most common patterns. The positive rate of anti-TP antibody was significantly higher in HIV antibody indeterminate samples than general population control group and WB negative control group (10.91%, 12/110 vs. 1.77%, 4/226 and 3.64%, 4/110), compared with two control groups (χ(2)=13.627 and 4.314, P<0.05). The positive rate of AFP was significantly higher in HIV antibody indeterminate samples than general population control group (18.18%, 20/110 vs. 0.44%, 1/226, χ(2)=39.736, P<0.05), the different was not significant compared with WB negative control group (18.18%, 20/110 vs. 23.64%, 26/110, χ(2)=0.990, P>0.05) While no significant differences were found between HIV antibody indeterminate group and two control groups in terms of the positive rates of ANA, HBsAg, anti-HCV antibody or anti-HTLV antibody. Conclusions: The influencing factors that leading to nonspecific responses to indeterminate HIV antibody tests appeared complicate, and the anti-TP antibody positivity might be an influencing factor responsible for nonspecific responses to indeterminate HIV antibody tests.
