Prevalence of drug resistance in treatment-naive HIV infected men who have sex with men in Guangzhou, 2008-2015.
10.3760/cma.j.issn.0254-6450.2018.07.021
- VernacularTitle:广州市2008-2015年未接受抗病毒治疗的男男性行为人群艾滋病病毒感染者耐药分析
- Author:
Z G HAN
1
;
Y L ZHANG
2
;
H WU
3
;
K GAO
3
;
Y T ZHAO
3
;
Y Z GU
3
;
Y C CHEN
3
Author Information
1. Department of Operational Control, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
2. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China.
3. Department of AIDS Control and Prevention, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
- Publication Type:Journal Article
- Keywords:
Drug resistance;
HIV;
Men who have sex with men
- MeSH:
Adolescent;
Adult;
Child;
Drug Resistance, Viral;
Genotype;
HIV Infections/ethnology*;
Homosexuality, Male/statistics & numerical data*;
Humans;
Male;
Phylogeny;
Prevalence;
RNA, Viral/genetics*;
Sexual and Gender Minorities;
Young Adult
- From:
Chinese Journal of Epidemiology
2018;39(7):977-982
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand the prevalence of drug resistance in treatment-naive HIV infected men who have sex with (MSM) in Guangzhou. Methods: HIV-1 RNA were extracted from the serum specimens of the MSM newly confirmed to be HIV-1 positive, living in Guangzhou and receiving no anti-viral therapy from 2008 to 2015. HIV-1 pol gene segments, including full protease and part reverse transcriptase, were amplified by nested reverse transcription polymerase chain reaction (nested-PCR) and sequenced by Sanger. Subsequently, the sequence data were submitted to Stanford University HIV Drug Resistance Database for drug resistance analysis. Results: Among 2 283 HIV infected MSM, HIV-1 pol gene segments were obtained from the serum samples of 1 986 HIV infected MSM aged 16-84 (30.18±8.24) years. Among them, the unmarried accounted for 74.17% (1 473/1 986), those of Han ethnic group accounted for 90.64% (1 800/1 986), those with education level of college or above accounted for 49.65% (986/1 986), those with education level of senior high school or secondary school accounted for 27.14% (539/1 986), those with education level of junior high school or below accounted for 20.89% (415/1 986). The distribution of subtypes was predominated by CRF07_BC (38.22%, 759/1 986) and CRF01_AE (34.49%, 685/1 986). The overall prevalence of drug resistance was 3.32% (66/1 986). The prevalence of resistance to protease inhibitors (PIs), nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs) were 1.36%(27/1 986), 0.65% (13/1 986) and 1.61% (32/1 986), respectively. Subtype B had higher resistance to PIs, NRTIs and NNRTIs and subtype CRF55_01B had highest resistance to NNRTIs compared with other subtypes. In subtype B, the resistant rates to D4T, EFV and NVP were highest (all 4.17%, 5/120), followed by those to NFV, AZT and RPV (all 3.33%, 4/120). In subtype CRF55_01B, the resistant rates to EFV and NVP were highest (all 5.50%, 16/291), followed by those to ETR and RPV (all 5.15%, 15/291). Conclusions: The prevalence of drug resistance in treatment-naive HIV infected MSM in Guangzhou remained at low level and current antiretroviral drugs are generally effective. However, subtype B and CRF55_01B have higher drug resistance.
